With a midnight deadline looming and hundreds of city jobs on the line, one more Chicago employees union has blinked while two others continue their stare-down with Mayor Daley.

Laborers Union Local 1001 signed a two-year deal Monday agreeing to the same cost-cutting measures that 40 other unions have also accepted, such as 24 unpaid furlough days a year, comp time instead of overtime pay, and unpaid city holidays.

“To keep the 300 people working, it’s something we’ll have to live with,” Laborers Local 1001 secretary-treasurer Lou Phillips told the Chicago Sun-Times.

According to the Sun-Times, Daley was prepared to reduce 100 more garbage collection crews from two laborers per truck to one if a deal with the union could not be reached.

A spokesman for the two hold-out unions, Teamsters Union 726 and AFSCME Council 31, told Chicago Public Radio that its members are some of the lowest paid city workers and can’t afford to take furlough days.

AFSCME leaders on Monday released a letter they sent to Daley asking the mayor for an agreement that doesn’t include laying off city workers.

“Despite the mayor’s claims to the contrary, his staff has refused — including as recently as a few hours ago — to meet with the union to discuss any alternative to layoffs since AFSCME members voted overwhelmingly to reject the Mayor’s initial demand that they take 46 unpaid days over the next two years, equal to a 10 percent pay cut,” read the letter from Henry Bayer, executive director of AFSCME Council 31.

At a Tuesday press conference Daley, flanked by labor leaders, urged the hold-outs to concede or face 431 layoffs Wednesday.

“If we do not come to an agreement by midnight, then tomorrow will be the last day on the city payroll for 431 employees represented by the Teamsters and AFSCME,” the Tribune reports.